The following entries for uhlsport Hellenic League Division Two have been made for the 2023/24 season. The four divisions (North, East, South, West) will be released shortly.
- Ardley United Development
- Aston Clinton Sports
- Bishop Cleeve Development
- Bourton Rovers Development
- Britwell
- Chalfont Wasps
- Chalvey Sports
- Cheltenham Saracens Dev
- Chinnor
- Cinderford Town Development
- Didcot Town Development
- Easington Sports Development
- Evesham United Development
- Fairford Town Reserves
- FC Beaconsfield
- Gloucester City Development
- Hazlemere Sports
- Headington Amateurs
- Highworth Town Reserves
- Holmer Green Development
- Kidlington Reserves
- Letcombe
- Longlevens Dev
- Mortimer
- Newent Town Reserves
- North Leigh Development
- Old Bradwell United Development
- Oxford City Development
- Prestwood
- Shipston Excelsior
- Swindon Supermarine Dev
- Taplow United
- Thame United A
- Tuffley Rovers Reserves
- University of Bristol
- Wantage Town Development
- Watlington Town